In Vitro Propagation Of Satureja Punctata (benth.) Briq. A Rare Aromatic And Medicinal Plant From Ethiopia Using Shoot Tip Explants

Satureja punctata is an important medicinal plant in Lamiaceae family. This plant is threatenedrnmainly due to overgrazing, deforestation, over harvesting of the whole plant for medicinalrnpurpose. This study was aimed to develop an efficient in vitro propagation protocol for S.rnpunctata using shoot tips. In vitro germinated seedlings were used as mother plant for culturerninduction. Shoots excised from thirty-day-old seedlings were cultured on Murashige and Skoogrn(MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) andrnKinetin (KIN) for shoot induction and multiplication. Half strength MS medium containingrndifferent concentrations of Indole-3- butyric acid (IBA), Indole-3-acetic acid (IAA) and α-rnnaphthalene acetic acid (NAA) were used for root induction. BAP at 0.5mg/l concentration andrnplant growth regulator free medium induced the highest frequency (100%) of shoot induction.rnMaximum mean number of shoot per explant (8.25±1.64) and shoot length (2.62±017 cm) werernobtained on medium containing 0.5 mg/l BAP and PGRs free medium respectively. Therncombination of BAP with NAA found to be an optimum concentration producing 26.20±2.71rnshoots per explant and 2.48±0.13 cm shoot length was obtained from PGRs free medium forrnshoot multiplication. MS medium supplemented with 1.0 mg/l IBA gave the highest rootingrnpercentage (100%) with optimum root number (5.90±0.48) and root length (1.55±0.11cm). Uprnon acclimatization, 97.5% survival rate was observed on soil composed of garden soil, sand andrnfarm yard manure at a ratio of 2:1:1, respectively. The results demonstrated that, this study wasrnvery important for mass propagation and ultimate conservation of this valuable plant.rnrnKey words; Acclimatization; in vitro propagation;, medicinal plant; plant growth regulators;rnSatureja punctata

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