Conversion of animal waste to biogas energy to replace traditional fuel and use of the slurry asrna fertilizer is the current focus of the national biogas program of Ethiopia (NBPE). This paperrnpresents the experimental results of the anaerobic digestion of Justicia schimperiana (Hochst.exrnA. Nees) T. Ander (JS) and cow dung each separately and with their various combinations atrnAddis Ababa University Environmental Science laboratory. The biomass of JS and cow dungrnwere characterized and then estimation of biogas production and methane content of each of therntreatments, T1 (cow dung alone), T2 (1:1), T3 (2:1), T4 (3:1), T5 (JS alone), T6 and T7 (withrndigester effluent) was performed by using indirect (water displacement) and absorption of CO2rnby 10 % NaOH methods, respectively. It was found that T5 (JS alone) was highest in the amountrnof biogas production but lowest in its quality and T3 (2:1 ratio of cow dung to JS) was the secondrnhighest in the amount of production, but the highest in quality. Thus, T3 produced the optimumrnmethane gas among treatments. Moreover, JS and its combinations with cow dung producedrnhigher volume of biogas and contain more macro-nutrient in the slurry for plants than cow dungrnalone. Thus, JS appears to be good material for biogas and bio-slurry production