An Ethnobotanical study was conducted to document medicinal plants used by Local people andrntheir indigenous knowledge in Machakel District, East Gojjam Zone of the Amhara RegionalrnState. The study was conducted by involving 72 informants that were randomly selected from 12rnselected kebles, from 72 (12 of them key informants). Ethnobotanical data were collected usingrnsemi-structured interview, field observation and group discussions. Data gathered were analyzedrnusing descriptive statistics, preference ranking, direct matrix ranking, informant consensus andrnfidelity level. In this study, From 82 species,57 species were used for the treatment of onlyrnhuman health problems, 9 species are treated live stock ailments and 16 species are treated bothrnhuman and live stock ailments.Asteraceae, which contributed 8 species, stood first followed byrnLamiaceae. Most of the medicinal species were collected from the wild. 39 (47.5%) herbs, andrnthe least were climbers 4 (4.8%) species.The most frequently used plant parts were leaves (35%)rnfollowed by roots (22.1%).The most frequent mode of preparation of plant medicine wasrnpounding (34%) followed by powdering (29%). The most common route of administration wasrnoral (37.6%) followed by dermal (34.1%). Fidelty level value showed that the most frequentrndiseases in the area are stomach ache, common cold and Evileye. In the study area Echinopsrnkebericho was the mostscarce plant.Ranking of six species of plants showed that Ocimiumrnlamifolium is the most preferred species by traditional healers for the treatment of Febrileillness.rnEuclayptus globulus was the most multipurpose species. Medicinal plant in the area is threatenedrnby agricultural expansion. Documenting the collected plants and associated indigenousrnknowledge can be used together with modern sciences for developing management plans forrnconservation and sustainable use of medicinal plants in the area.