The Effects Of Crude Extracts And Fractions Of Alchemilla Abyssinica On Smooth Muscle Of Guinea Pig Ileum

Alchemilla abyssinica is a plant widely used in traditional medicine. Its wide use among therncommunity plus already established scientific evidences for medicinal values of other Alchemillarnspecies provided good ground for this investigation. In this research, CHCl3/EtOAC 1:1 extractrnof dried aerial parts of Alchemilla abyssinica, methanolic extract of the CHCl3/EtOAC residuernand fractions of the methanolic extract were tested on isolated guinea pig ileum (GPI) forrnpossible presence of spasmogenic (contractile) or spasmolytic (relaxant) effects. Concentrationsrnof each extract and fraction ranging from 20-600 μg/ml final organ bath concentration wererntested. The effects of these test samples on the basal rhythmic contractions of the GPI as well asrnon its contraction elicited using the agonist, histamine, were determined. The antagonist,rnPapavarine, was also used as a control smooth muscle relaxant. While the CHCl3/EtOAC 1:1rnextract showed neither spasmogenic nor spasmolytic result, the methanolic extract showedrnmarked spasmolytic effect. This methanolic extract was fractionated using columnrnchromatography and the fraction eluted using Hexane/EtOAc 1:2 gave greatest spasmolyticrnresult and it was taken as the final test fraction. The final test fraction produced significantrn(P

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