Quantitative Determination Of Carbazole Alkaloids From Crude Plant Extract (glycosmis Pentaphylla Of Family Rutaceae) Using Gas Liquid Chromatography

Quantitation of carbazole alkaloids (Carbazole, 3-MethylCarbazole.Glycozoline and Glycozolidine) was carried out usingGas-Liquid Chromatography (GLC ). This was -the first time aquantitative work was undertaken on carbazole alkaloids©A stock solution of 1mg/ml in chloroform was prepared ofeach of the above mentioned carbazole alkaloids© Appropriateworking concentration were prepared by suitable dilutions ofvarious stock solutions .with chloroform. Heptazolidine ,(lmg/ml) in chloroform, was used as the internal standard (i.s.)The plant extract (Glycosmis Pentaphylla of family Rutaceas)solution (unknown sample) was prepared by drawing a drop(weighed 23mg) from the light Petroleum extract by using aclean glass rod and diluting it to 5ml with chloroform. TheI.S. (1ml) was added to all solutions prior to analysis© Allsamples (3ul) were chromatographed on a Bye Unicam series 204chromatograph equipped with FID and Temperature programmer©A single point internal standard method was employed for theanalysis of result. TLG was performed of carbazole, 3 - methylcarbazole and the Crude plant extract©Single point method employed was faster and moreconvenient that the traditional individual calibration curvemethod. The concentrations of the alkaloids were found as:-1. Carbazole - 17.35 ug/ml (0.4% w/w)2. 3-Methyl Carbazole - 186.67 • (4.06% * )4. Glycozolidine - 189.70 " (4.1 2% " )plantTLC results added supportive evidence that the/extractcontains carbazole and 3 - methyl-carbazole (all identifiedfor the first time from this plant species)c G-LC was foundto be a very convenient means of finding the levels of componentsin a plant extract.

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