Modulatory Role Of Resveratrol-induced Dietary Restriction And Environmental Enrichment On Neurobehavioural Outcome In Young Healthy Mice

Dietary Restriction (DR) otherwise known as Caloric Restriction (CR) has been generallydefined as consumption of nutritious diet that is 30% to 40% less in calories compared toad libitum diet while Environmental Enrichment (EE) is defined as a sustained andprogressive increase in cognitive and sensorimotor stimuli with aggregated voluntaryphysical activity and complex social interactions. The aim of this experiment was toinvestigate the modulatory role of Resveratrol induced CR and EE on neurobehaviouralresponses in young healthy mice. Twenty five mice of both sexes were divided into fivegroups of 5 animals each: group I served as the control and receivedcarboxymethylcellulose (CMC) 50 mg per kg/day orally, group II animals were maintainedon every other day feeding, group III animals received Resveratrol 50 mg/kg, suspended in10 g/L of (CMC) orally per kg/day. Group IV animals received CMC and kept in anEnriched Environment while group V animals received Resveratrol 50 mg/kg and kept inan Enriched Environment. The treatment lasted for the period of four weeks. On day 26,27 and 28 of the study period, the animals were subjected to neurobehavioural evaluationof motor coordination, motor strength, learning and memory. Brain and plasma sampleswere evaluated for lipid profile, lipid peroxidation and antioxidant enzymes. The resultsshowed significant increase (P < 0.05) in the transfer latency (acquisition) in the everyother day feeding group (69.20 ± 9.03 seconds) compared to the control group (36.80 ±5.58 seconds). A significant increase (P <0.05) was observed in the concentration of lowdensity lipoprotein (LDL) in every other day feeding group (1.72 ± 0.12 mg/dl) andenvironmental enrichment treatment group (1.73 ± 0.16 mg/dl) when compared to thecontrol group (1.11 ± 0.07 mg/dl). The results obtained also showed significant decrease < 0.05) in MDA concentration in the Resveratrol treatment group kept in EE (1.50 ± 0.05IU/L) compared to the control (1.84 ± 0.09 IU/L) and GPx activity in the Resveratroltreatment group kept in EE (41.00 ± 2.02 IU/L) compared to the control (59.00 ± 2.85IU/L). In conclusion, the results obtained demonstrated that Resveratrol induced CR andEE have no effects on neurobehavioural responses coupled with some significantalterations in lipid profile, lipid peroxidation and antioxidant enzymes in young healthymice over a period of four weeks.

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