In Vitro Effecacy Of Methanolic Extracts Of Vernonia Amygdalina Croton Macrostachyus Ricinus Communis And Petroleum Ether Extract Of Millettia Ferruginea Against Bovicola Ovis And Rhipicephalus (boophilus) Decoloratus

In vitro loucicidal and accaricidal efficacy evaluation of Vernonia amygdalina, Croton macrostachyus, Ricinus communis and Millettia ferruginea against Bovicola ovis and Rhipicephalus decoloratus of cattle were carried out from January 2014 to April 2015. Crude methanol extracts of Vernonia amygdalina, Croton macrostachyus, Ricinus communis leaf and Petroleum ether extract of Millettia ferruginea seed oil were prepared for in vitro test at different time intervals. The four selected medicinal plants, at concentrations of 200, 100, 50, 25, 12.5 and 6.25mg/ml and a commercially used acaricide (0.1% diazinon), were examined using in vitro adult immersion test. In vitro loucicidal test showed all concentration of Millettia ferruginea oil (μl/ml), ≥25mg /ml concentration of Croton macrostachyus and 200 and 100mg/ml concentration of Ricinus communis had pronounced loucidal activity at 24hr of exposure. 200mg/ml concentration of Vernonia amygdalina and ≤6.25mg/ml concentration of Croton macr ostachyus had moderate loucicidal activity at 24hr of exposure. Moreover, the 200 and 100μl/ml concentration of Millettia ferruginea seed oil and 200mg/ml concentration of Ricinus communis leaf crude extract had high accaricidal activity (90-100%) against Rhipicephalus decoloratus while crude methanolic extracts of Croton macrostachyus and Vernonia amygdalina and even the positive control (diazinon) showed low acaricidal activity (

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