BACKGROUND: Ventilator-associated pneumonia (VAP) continues to be a common and potentially fatalrncomplication of ventilator care and often encountered within intensive care units (ICUs). Ventilated and rnintubated patients present critical care Health care workers the unique challenge to incorporate evidence-basedrnpractices surrounding the delivery of high-quality care. This study examined the Assessment of Knowledge andrnpractice of Critical Care HCWs towards Ventilator Associated Pneumonia Bundles in TASH adult ICU rnOBJECTIVE: to Assess Knowledge and practice of Critical Care HCWs towards Ventilator AssociatedrnPneumonia Bundles inTASH adult ICU rnMETHODS: Cross-sectional study was conducted from September to November 2020 among 160 HCWrnAddis Ababa University TASH working in adult ICU who were selected by a stratified sampling technique. a rnstructured questionnaire was used to collect data. Data were entered and analyzed using SPSS version 26 andrnthe results was presented by tables, bar graphs and pie chart. Variables with p-value < 0.05 were used asrnsignificantly associated with the dependent variable. rnRESULTS: Most (67.5%) of the study participants were male and 70.6 % were ages 25-30 years. Most ofrnthe respondents were residents (69.4%) and had a work experience below 5 years (73.1%). The total mean rnknowledge score of the participants was 12.4312 out of 16 (Range 5-16, SD= 2.10606). 51.9 % of thernparticipants had scores above the mean (i.e. good knowledge score) while the rest had below the mean scorernwith only 155 (96.9%) of the study participants scoring above 8 out of 16. The mean practice score of thernrespondents was 14.9625 out of 25 (Range 7-23, SD = 3.48562). Fifty three point one percent (53.1 %) of thernrespondents had a mean practice score above the mean (good practice) while the rest had poor practice. 25rn(15.6 %) respondents had total practice score below 12 out of 24 (i.e. below 50%). rnCONCLUSION: In this study, around half of HCWs have good knowledge (i.e. 51.9%) and around half ofrnHCWs have good practice (i.e.53.1%). The percentage of HCWs who score good practice is higher than who rnscore good knowledge. No significant association between age, gender, working department, years of workingrnexperience and knowledge. It was profession (job title) that had statistically significant impact on having goodrnknowledge score. Practice of ICU HCWs on VAP prevention bundles was not statistically associated with age,rngender, working department, years of working experience and profesion.but nurses had higher odds of havingrngood practice. There was no correlation between knowledge and practice score.