Background: The COVID 19 was announced to be a worldwide pandemic by the WHO on Marchrneleven, 2020. It introduced serious work-related health risks to the HCPs owing to their frequentrnexposure to infected persons. Protection of HCPs and prevention of intra-hospital transmission ofrninfection are important aspects in epidemic response and this requires that HCPs must have updatedrnknowledge regarding the source, transmission, symptoms, and prevention of COVID-19. Having poorrnknowledge, poor practice and negative attitude toward the disease among HCPs could result in a negativernimpact in the supportive treatment and, worsen the spread of the pandemicrnOBJECTIVE: The main purpose of this study was to assess Knowledge, Attitude, and PracticernRegarding Covid-19 Transmission among Health Care Workers who work in TASH Operating Theater.rnMethods: Cross-sectional study was conducted on September 2020 among 261 HCW who were selectedrnby a stratified sampling technique. Pretested structured questionnaire was used to collect data. Data werernentered and analyzed using SPSS version 26. Variables with a p-value < 0.05 were used as significantlyrnassociated with the dependent variable.rnResult: A total of 261 HCW had participated in the study with a 100% response rate. The majority of thernrespondents were male (69.7%) with a median age of 30 (range 23-63) years. The mean knowledge scorernwas 81.11 % (i.e. 11.3554 out of 14 with SD=1.52). The mean attitude score was 68 %( i.e. 6.8 out of 10rnwith SD=1.32) with sixty-five percent point five (65.5%) of the respondents have an overall positivernattitude. the occupation was the only variable significantly associated with knowledge and attitude. Therntotal mean practice score is 45.36% (i.e. 4.08 out of 9 SD= 1.6); with no statistically significantrnheterogeneity across socio-demographic, IP training status, knowledge, and practice. Most (54.8 %) ofrnthe respondents scored below the mean (45.36 %) rnConclusion: In this study, around half of HCWs have good knowledge and two-third of HCWs have arnpositive attitude score towards COVID-19 transmission. The total mean practice score and those whornscored above the mean are both below 50% which shows having poor practice. The occupation was thernonly independent variable with a significant association with knowledge and attitude.