Bioremediation being a problem solver for many different environmental and other majorrnhazards, they play an important and cost effective role in the decaffeination process. Formrncollected environmental sample coffee husk. From CH caffeine can be eliminated. With thisrnexperimental work take two spp of microorganism that were bacillus subtilis and rhizobium fromrnEthiopia bio diversity institute, that can be capability of improving soil fertility. Could grow onrnthe medium supplemented with 2.5g/L caffeine and could effectively degrade 2.5 g/L of caffeinernin the liquid media as a sole source of carbon and nitrogen. Morphological and biochemicalrncharacteristics were maintained for re-affirmation the organism as bacillus subtilis andrnrhizobium. The degradation of caffeine capability was authenticated by growth curve. The nextrntask were that those authenticated caffeine degrading bacteria applicable for conserving soilrnfertility by means of N-fix and p- solubilized potential then propagation and growth of thosernselective caffeine degrading bacteria was maintained by selective medium with and withoutrnsupplement of 2.5g/l caffeine then optimized the factors that affecting cell growth with preparedrnbroth with and without supplement of caffeine and determined interaction of each factor fromrnexperiment design software 6.0.8) the result would be temp; 35rnornc, pH 7.07 incubation time 36hr rnfrom those optimal condition obtained maximum cell growth in number was 3.41995E+008rnand 5.0707E+008 with and without caffeine respectively for bacillus subtilis and temp 34rnornc, pH rn6.6 and incubation time 28hrs optimum condition maximum cell growth 3.51146E+008 andrn4.09131E+008 was obtained for rhizobium sp. of organism. That viable cell grownrnmicroorganism ready for applicable of as liquid bio fertilizer those were easiest way ofrnproduction, handle, cost effective and keep soil health care method of production.