Molecular Characterization Of Introduced Sugarcane (saccharum Officinarum L.) Genotypes In Ethiopia Using Inter Simple Sequence Repeat (issr) Markers

The major thrust of sugarcane (Saccharum officinarum L.) variety improvementrnprograms is to increase sugar yield, while due to its genetic complexity, sugarcane hasrnreceived very little research interest, despite its economic importance, and molecularrntechniques are being developed only in recent times. Furthermore, until the present daternno molecular studies were carried out to evaluate sugarcane germplasm from Ethiopianrnfields. Therefore, the objective of this study was to evaluate genetic diversity andrnestablish relationships among populations of sugarcane in Ethiopia using ISSR markers.rnGenomic DNA was extracted from leave samples of 82 sugarcane genotypes according tornmodified CTAB method. A total of 149 scorable and reproducible bands were generatedrnusing 12 ISSR primers among which 124 were polymorphic and attributed to PPL =rn83.22 %, h = 0.31, and I = 0.45 at species level. Intra-population diversity based onrnpolymorphic loci ranged from 28.86 % to 47.65 % with mean of 38.35 %, Nei’s generndiversity of 0.097 to 0.171 with mean of 0.137, Shannon’s information index of 0.147 torn0.255 with mean of 0.205, and within population AMOVA of 63.56 %. All diversityrnparameters confirm that, the highest diversity was obtained from those that were obtainedrnfrom France and Cuba whilst the lowest was from those of Barbados and South Africa.rnAMOVA revealed 36.4 % among population variation which is significantly lower thanrnthat of within population variation. From Jaccard’s pairwise similarity coefficient, thosernfrom Sudan and South Africa were most related populations exhibiting 0.70 similarityrnand USA and Barbados were the most distantly related populations with similarity ofrn0.53. With all clustering analysis, most of the genotypes clustered to their respectivernorigins. Thus, ISSR markers detected a range of diversity from sugarcane varieties withrntheir unique identity that deserve conservation attention and improvement programs.rnrnKey words: Commercial crop, genetic diversity, ISSR markers, Saccharum officinarum

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