Morphological And Molecular Characterizations Of Wilt Disease Of Ginger (zingiber Officinale Roscoe) Caused By Fusarium Sp

Ginger rhizome, pseudostem, leaf and soil samples were collected from ginger growingrnareas of southwestern Ethiopia. Fungal pathogens were isolated from ginger partsrnshowing vascular browning symptoms and soil following standard methods. Isolationrnrevealed fungal isolates belonging to four genera: Fusarium, Penicillium, AspergillusrnandTrichoderma. Out of 24 fungal isolates 14 isolates were Fusarium species. Inrnvitropathogenicity test resulted in pathogenicity index (PI) values ranging from 10.96%rn+1.55(AAUFG13) to 45.35% +11.57 (AAUFG6). Cultures of Fusarium species onrnPDAmedium showed white, creamy white, dull pink and pink coloration. Theyrnimparted dullwhite, orange, light reddish purple, intense reddish purple and darkrnreddish purple pigmentations on the riverse side. Canoe shaped macroconidia, ovoid microconidia and short phialides were pertinent to all of the Fusarium isolates. Based on their cultural and morphological characters the isolates were identified as Fusarium oxysporum. The ability of the isolates to induce rhizome rot confirmed the forma specials rank of the isolates asrnF. oxysporum f. sp. zingiberi (Foz). PCR- RFLP profile of the ITS1 rDNA regionrnindicated genetic variation between the Fusarium isolates. In silco RFLP digestion ofrnITS1 rDNA sequences of F. oxysporum retrieved from the fungal ITSoneDB wasrnperformed using online restriction mapper software and restriction enzymes used in thernactual experiment. The result of the in silco restriction digestion simulation was similarrnwith the actual PCR_RFLP study supporting that the isolates are Fusarium oxysporum.rnrnKey words: Fusarium wilt; ITS1;Pathogenicity; PCR-RFLP; Rizhome rot

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