The reaction of gossypol vuth 3~anuno-l~propauol and iron (III)rnin acidic medium produces a green colored complex soluble in isopropylrnalcohol-fcexa.no mixture (3:2), having a characteristic absorptionrnmaximum at 6"o ran, The co-ored reaction product, is sufficiently stablernand obeys Beerâ€™s lav: in the concentration range 5 -84 ppn of gossypol,rnTlie effective molar absorptivity in terms of goegypol is found to hern-1 -1rn64f% 1. mole . cm. vdth a photometric sensitivity of 0.08;jg ofrnyrngossypol,per an. On tlie basis of sensitive color reaction, a newrnmethod, has been developed for the determination'.of gcsaynol, The ,rneffect of several experimental variables on the determination ofrngossypol have been studied. The stoichiometric composition of therngreen colored goseypol^aminoprcpanol-iron (III) complex has beenrndetermined and was found to be 1:2 (gossypol. to iron) The precision of the method has leon evaluated in terms ofstandard deviation and the relative standard deviation vjarsfound tobe + o.71%.The validity of the method was established by the reccwery ofrnstandard gosaypol added to the cottonseed samples,rnThe proposed motljod has been applied for analysis of totalrngossypol in several varieties of cottonseed grown under differentrnconditions in Ktliiopia and tho cottonseed press cakes available in.rnthe market. The goravrol content of the samples were detorrdnedrnafter extraction of total gossypol from tlie cottonseed and cottonseedrnpress cakes by 3~oriinoâ€œ l~propanol in dimethylforrtvoride. The resultsrnobtained by the proposed method liavc leon compared. with the resultsrnobtained by standard A.O.O.S. method. Tho tvjc results were found tornbe almost identical.