Chemical Composition Of Croton Macrostachys
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The root~·bark powder of Croton macrostachys Hochstex. A.rnRich. (Euphorbeaceae) (ብሳና), used in Ethiopia for curingrnspreading wounds, was extracted with diethyl ether. The crudernextract, on fractionation by column chromatography (Silica gel)rnyielded a major solid compound A (mp 210'-212o) crystallizablernfrom acetone~methanol (9:1) into beautiful needle aggregates,rnthe structure of -Ihich was identifted, using IR, PMR and MSrndata, to be that of lupeol, a known antitumor agent. Therntumor inhibitory compound Crot-poxide which was previouslyrnisolated from the fruits could not be detected in the root~rnbark extract. Preliminary investigation also showed thernpresence of trace solids Band C having the same Rf on TLC.rnB was water isoluble .,hi te solid while C was water solublernand sho~led strong blue fluorescence under UV-lamp. Thernpresence of quaternary alkaloid was also confirmed in thernroot-bark.
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