The objective of this study is to further expand the use of Remote Sensing and GISrntechniques in the country, especially in the area of project planning, monitoring,rnevaluation and impact assessment in the agriculture sector. Impact assessment ofrnthe Southern Tsetse Eradication Project (STEP) was carried out using RemoternSensing and GIS techniques, by taking Humbo Woreda (SNNPR) as a case study.rnLandsat ETM+ image of the area was used and agricultural sample survey wasrnconducted in 11 peasant associations in the study area. Pertinent questionsrncategorized into household characteristics, agricultural data, tsetse fly andrntrypanosomiasis condition as well as environmental related issues regarding pre andrnpost project periods were included in a structured questionnaire. Statisticalrncomparison of with and without project situation of various parameters was made.rnThese include size of cultivated land, amount of crop production, method ofrncultivation, number of livestock, oxen out put, situation of tsetse infestation andrntrypanosomiasis prevalence and environmental situations in the area, etc. Surveyrndata was manipulated and spatially presented by creating a spatial database in GISrnsoftware and using spatial facilities such as grid interpolation, proximity analysis,rndensity calculation, map query, grid analysis and layout facilities.rnThe project had no socio-economic baseline data thus, farmers' recall method wasrnapplied as alternative source of information. In addition to this, due to the absence ofrnrecent satellite image, it was not possible to compliment the agriculture samplernsurvey with remote sensing data. As a result it was not possible to assess the landrnuse! cover and environmental change analysis at the desired level.rnAssessment based on the available information, showed that the so far undertakenrntsetse control intervention by STEP has enabled to significantly reduce the tsetsernand trypanosomiasis problem in the area. As a result, livestock health condition hasrnimproved while, livestock body and growth condition have not shown similarrnimprovement mainly due to feed and water scarcity. There has been a clear shift fromrnhand cultivation to the use of oxen power while average cultivated size andrnproduction of major cereals that normally require oxen power for their CUltivation has increased. The impact is more visible in kola areas, probably due to the f act thatrnthese areas had been highly affected by the problem and where most impact isrnexpected as a result of the control intervention.rnThe application of advanced survey and monitoring tools, including remote sensingrnand GIS, has in general, allowed appropriate situation analysis. Finally, it wasrnrecommended that any project need to undertake baseline as well as regularrnmonitoring surveys enabling data collection for future project impact assessmentsrnand the use of recent satellite image for detailed land use! cover change analysis.rnKey words: STEP, Impact Assessment, Remote Sensing and GIS, Tsetse fly,rnTrypanosomiasis, Apparent Fly