Production Of Bacterial Amylase And Evaluation For Starch Hydrolysis

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In starch Degrading industry, the first step to be preformed is gelatinization of starch granules atrnelevated temperature. This process tends to form a viscose suspension, thus making mixing andrnpumping a huge challenge. Therefore, to lower viscosity of starch paste thermo stable enzymes arernadded for breaking down the polysaccharides into oligosaccharides by a process known asrnliquefaction. Therefore, availability of these enzymes can be considered a prerequisite to start arnsuccessful starch liquefaction and saccharification industry. The main objective of this study was,rntherefore, to produce efficient microbial amylolytic enzymes for starch hydrolysis. One bacterialrnisolate designated as AAU 2107 was isolated from locally collected samples. The enzyme has anrnoptimum activity at pH of 5.0 and 70°C. Addition of calcium in the reaction mixture was shown tornstabilize the enzyme. The isolate AAU 2107 was able to grow under solid state fermentation (SSF)rnusing wheat bran as a solid substrate. Optimum enzyme production was recorded at 32°C, and atrn120 h of incubation and at a moisture content of 75%. Addition of peptone to the SSF medium asrnan organic nitrogen supplement enhanced enzyme production. The enzyme was used to hydrolyze arn25 % (w/v) of starch at 70°C and pH 5.0, and the resulting hydrolysate was analyzed on TLCrnplates. After 4 h hydrolysis, the TLC chromatogram showed release of glucose, maltose,rnmaltotriose, and other oligosaccharides, indicating that the enzyme is endo acting alpha amylase.rnThe results of this study show that amylase from AAU 2107 has a good potential to be used inrnstarch liquefaction and saccharification processes. The ability of the isolate to grow and producernthe enzyme under SSF using cheap agro industrial wastes could help to greatly reduce thernproduction cost of the enzyme.

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Production Of Bacterial Amylase And Evaluation For Starch Hydrolysis

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