Introduction: - Healthcare Associated Infection (HCAI) is the major cause of morbidity andrnmortality in hospital based settings; with nosocomial infections and their control creating arnworld-wide challenge. This also adds to the burden of healthcare costs due to addedrnantimicrobial treatment and prolonged hospitalization. Para medical staff, especially nurses is atrngreat risk of exposure to blood and body fluids and other means of spread of infection.rnObjective: - This study was conducted to access knowledge, attitude and practice regardingrnstandard precautions among the nursing staff work in emergency and ICU at Tikur AnbessarnSpecialized Hospital. Method: - A cross sectional study was conducted on knowledge attitudernand practice of nurses working in emergency and ICU at Tikur Anbessa Specialized Hospitalrnfrom December 2016 to Jun 2017 GC. Data were collected using pre tested questionnaire on 142rnnurses selected by convenience sampling method. Collected data were checked, coded andrntransferred to SPSS version 22 for analysis. Frequency, Mean and other statistics wererncalculated. Result: - This study revealed that, the mean knowledge score of all participants wasrn5.65, i.e. more than half (60.5%) of the studied sample had good knowledge level. The meanrnattitude score of all respondents was 4.95 which showed that 55.6% of the studied participantsrnhad good attitude level. However, only 48.59 % of the participants had Good practice with arnmean score of 6.34. According to the correlate bivariate model No significant statisticalrncorrelation were found between mean Attitude scores towards age, years of experience, genderrnand factor affecting SP. Significant statistical relation were found between mean knowledgernscores and year of experience (P=0.02). And a significant statistical correlation were foundrnbetween mean practice scores towards year of experience, gender, and factor affecting SPrn(P=0.02, 0.01, 0.04) respectively. Conclusion:- Based on findings of the current study, it can bernconcluded that in spite of having positive attitude level regarding standard precaution, nurses hadrnfair knowledge level and poor practice level. Low compliance of hand hygiene, lack of training,rnheavy workloads, lack of infection prevention supply, unavailability of guideline, lack ofrnawareness about availability of PEP and shortage of vaccine for HBV are major obstacles forrncompliance of standard precaution. Recommendation: - Providing training on standardrnprecaution to all health care staff and enhancing sustainable supplies systems are recommended.