Natural dyes have gained importance due to the growing environmental awareness andrnimplementation of stringent regulations in production and use of synthetic dyes. This scenariornnecessitated the present investigation on bio-dyes Therefore, to address some of these issues arnstudy was conducted to produce natural dye and evaluate the efficiency of dyeing on sheeprncrust. Flower of B. macroptera and leaves of E.camaldulensis were extracted with n-hexane, nhexane:rnchloroform 1:1, methanol: chloroform 1:1and methanol. The dyes produced fromrnB.macroptera and E.camaldulensiswere analyzed by TLC, FTIR, ColumnrnChromatography,UV-Visible spectrophotometer and GC-MS.The colorrncoordinates(L*a*b*and Î”E*ab) and lead metal were determined by color meter and ICPOES,rnrespectivelyas well aswash, light, rub and perspiration fastness values were determined byrncolor fastness tester. The results of the study revealed that methanol extract showed goodyieldsrnof 34.3% and 32.9% for B. macroptera and E. camaldulensis, respectively. The Rf values forrnboth plant extracts were 54.4 and 52.2 for commercial dye. The FTIR result showed primaryrnamine C-N stretching group in plant extract as well as in commercial dye.rnSpectrophotometrically, the extracted dye and novocol dye showed yellow color withrnabsorption spectrum of 436nm, 449nm, 450nm for novocol, E.camaldulensis andrnB.macroptera, respectively. Color bearing compounds such as ellagicacid, quercetinand gallicrnacid (Benzoicacid,3.4.5-trihdroxy) obtained fromE. camaldulensisandlutein fromrnB.Macropterawhile6-Amino.1,4-diazoanthraquinone obtained from commercial dye. The dyedrnleather from plant extract were showed better color fastness propertiesand the rating wasrnrecorded from very good to excellent and freeof lead metal wereobtained . value of b*rn(yellowness, 52.82,49.2),a*(redness,21.7,11.2)and Î”E*ab(35.57,26.17)forB. macroptera andE.rncamaldulensisrespectivelywhich implies the B. macroptera produced yellowish orange and E.rncamaldulensis produced yellow color with full capacity of shading and fullness.The resultsrnshowed that natural dye from E. camaldulensis leafandB. macroptera flowers extract havernpotential applications for leather dyeing.rnKeywords:Natural dyes, Eucalyptus camaldulensis and Bidensmacroptera (Adey Ababa)