In this study the biogas production potential of cotton seed cake (CSC) and rapeseed cake (RC) were assessed at laboratory scale. The physico-chemical analysis data (TS, VS, C:N ratio and pH) of the cakes confirmed that RC and CSC are feasible feedstock of anaerobic digesters. By blending RC and CSC with cow dung in different ratios the feedstock of the biogas digesters was prepared. From the nine treatments the maximum biogas of 6.1 L with 72% quality was generated by CP-5 digester (50% CSC & 50% CD) followed by CP-1 digester (50% RC & 50% CD) having 5.2 L biogas through 65% quality. The control (CD alone) produced 2.3 L biogas with 67% quality. Since the slurry of anaerobic digester contain essential plant nutrients; the content of major plant nutrients that are left in the digesters were measured at the end of retention time. CP-5 digester (the highest biogas producer) left with slurry of 3206.14 mg/L total nitrogen, 9707.88 mg/L of available phosphorus and 1238.85 mg/L of available potassium.rnKey words: oil seed cake, Biogas, slurry, bench scale biogas digester, macro nutrient