The objective of this study was characterization of dairy wastes (cheese whey) and its utilizationrnfor the production of bioethanol. The key to the utilization of this resource has been changing thernperception of cheese whey from a 'waste material' to an 'opportunity' for further processing. Dairyrncheese whey consisted of biological oxygen demand (BOD = 230mg/mL) and chemical oxygenrndemand (COD = 70mg/mL). It represents largely disaccharide sugar content lactose (0.036 â€“ 0.048rnmg/mL or 3.36 â€“ 4.48 %) and thereby, it is managed by converting into bioethanol with the helprnof yeast strain. In this work, kluyveromyces delphensis yeast strain was used for bioethanolrnproduction from cheese whey in batch fermentation (Erlenmeyer flasks). The composition ofrncheese whey was determined and characterized to identify its impacts on environment. Thernexperimental design was studied with central composite design (CCD) to investigate the effects ofrnsignificant factors including initial lactose concentration (4.5, 6.15 & 7.8g/L), yeast cellrnconcentration (5, 7.5 & 10g/L), temperature (27rnornC, 31rnornC & 35rnornC) and pH (5, 5.5 & 7) onrnfermentation process. The response surface methodology (RSM) was applied to investigate therninteraction effect of fermentation process variables and to achieve maximium yield of bioethanolrnfrom cheese whey. The best operating conditions were found to be initial lactose concentrationrn6.15g/L, yeast cell concentration 10g/L, temperature 27rnornC and pH 5.5. Under this condition, 15.5rnmL of bioethanol per 500mL of cheese whey was fermented as a final point. The maximum ethanolrnyield obtained from cheese whey was 20.4 %. The functional groups of cheese whey Bioethanolrnwere determined by using FT-IR with the help of IR correlation charts and moreover, therncharacteristics of bioethanol was investigated. This ascertained that the product obtained fromrncheese whey is definitely ethanol due to the confirmation of regions. Consequently, cheese wheyrnis a good resource for production of bioethanol.