Production of biogas through anaerobic digestion of organic waste materials provides anrnalternative environmentally friendly renewable energy. In this study, biogas production from codigestion ofrnbreweryrnwastewater, spentrnyeastrnandrnbreweryrnsludgerninrnfivernmixrnratiosrnwasrnevaluatedrnrnunderrnrnmesophilic condition (35 â„ƒ, 37 â„ƒ, 39 â„ƒ) using a batch digester for 21 days. For allrnsubstrates, total solids (TS), biological oxygen demand (BOD), chemical oxygen demand (COD),rntemperature (T), total nitrogen (TN), total phosphorus (TP), and pH were measured before andrnafter digestion. For the experimental design, different mix ratios of yeast and wastewater byrnvolume (0 %, 1 %, 6 %, 11 %, 100 %) were adopted for all the anaerobic digestion employed. Allrnmeasured physico-chemical parameters of the sample substrate significantly varied before andrnafter anaerobic digestion AD. Gas production was measured for all the samples periodicallyrnstarting from fourth day of digestion experiment and went to zero at about 21 days in all substrates.rnAssessment of cumulative biogas production revealed that substrate in a mix ratio of 1 %,rntemperature 37 â„ƒ, pH 6.5 (3417 mL/800 mL sample) spent and 6 %, 37 â„ƒ, 6.5 (3325.5 mL/800rnmL sample) showed the highest biogas production and the lowest was in 11 % volume ratio, 35 â„ƒrntemperature and pH of 5 (2173 mL/800 mL sample) and 11 %, 39 â„ƒ, 5 pH suggesting the firstrnmix ratio of the two substrates is an optimal mix to yield better biogas. Overall the results indicatedrnthat biogas yield and TS, BOD, COD reduction can be significantly enhanced when brewery spentrnyeast and brewery wastewater are co-digested.