In this study, NaOH/bentonite catalyst was synthesized and its performance was evaluated forrnbiodiesel production from cottonseed oil. Impregnation method was used for the synthesis ofrnNaOH/bentonite catalyst and the best catalyst was selected based on the yield ofrntransesterification reactio. The highest yield of biodiesel was obtained by using the catalystrnsynthesized at 500 rn0rnC of calcination temperature, 5 hr. of calcination time, and NaOH to rnbentonite ratio of 0.20. The result of the XRD and FTIR characterization showed that thernimpregnation of NaOH was successful and the active and basic component Na2O was obtainedrnduring calcination process. The optimization result showed that the optimum condition for therntransesterification reaction by using the selected catalyst was a temperature of 64.45rn0rnC, a catalyst rnloading of 2%w/w of cottonseed oil, and methanol to oil molar ratio of 6 which resulted inrn89.7423% biodiesel yield. Different physicochemical properties of biodiesel produced at thernoptimum parameters were determined and it was in the range of international standards. ThernFAME content of the produced biodiesel was 95.8%, which was above the minimumrnrequirement.