Process Performance Microbial Community And Composition Of Phased Anaerobic Digestion Of Heterogeneous Wastes For Bioenergy Production

The anaerobic mono-digestion and co-digestion(ACoD) of abattoir wastewater(AWW) andrnheterogeneous mixtures of fruits-vegetables solid waste(MFV) were studied using two-phasernanaerobic sequencing batch reactors(ASBRs). It was aimed at exploring the bioenergyrnrecovery potential of both substrates, and the influence of retention time(HRT), substrate type,rnand substrate mixing ratio on the performance and stability of the ASBRs. The HRTs were 7,rn5, 3, and 1 day for the acidogenic phase, and 21, 18, 15, and 12 days for the correspondingrnmethanogenic phase, i.e., 28, 23, 18, and 13 days of total HRT for the overall two-stagernASBR. The effect of co-substrates additions of both substrates to each other was investigated withrnfive different mixing ratios with and without agitation. The ASBRs were fed on a semi-continuousrnbasis and operated under mesophilic conditions at a lab-based experiment. Moreover, thernmetagenomics 16S amplicon sequencing of digestate samples was done to insight the microbialrncommunities of the methanogenic bioreactors using Illumina MiSeq. During the mono-digestion,rnboth HRT and substrate type significantly (P

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