The objective of this study was to analyze the Mediating role of service quality on the relationship between employee working environment and customer satisfaction: The case of kality dry port in Ethiopia. To achieve objectives of the study, established questionnaires was adapted from previous research works with modification and distributed to employees and customers of the port. Cross-sectional survey design was used to quantitatively analyze the relationship between the study variables. Simple random sampling technique was used to determine sample size from target population for customer respondents and Census was employed to employee respondents of kality dry port as the number of employees is too small. Therefore, mixed technique was used as each category of respondents was responded to different questionnaires. Based on valid data collected from 200 employees and customers of kality dry port, and from the overall analysis, it was established that the three work environment components considered in this study (Physical work environment, Training, and Reward) had positive and statistically significant effect on customer satisfaction. Service quality had a positive and statistically significant effect on customer satisfaction as evidenced by the study. In addition to that the three work environment components considered in this study (physical environment, Training, Reward) had a positive and statistically significant effect on service quality. Finally, the paper concluded that service quality partially mediated the relationship between the physical environment and customer satisfaction whereas; it fully mediated Training and Reward with customer satisfaction.rnKeywords: Kality Dry Port, Physical Environment, Training, Reward, Service Quality,rnCustomer Satisfaction, Mediation