A study was carried out on donkey milk in Adea woreda with particular emphasis onrncharacterization of the nutritional and microbiological properties of Abyssinian donkey milkrnfrom Adea woreda. The results were compared with those already published in literature for arnpotential future use as replacement of human breast milk. To this end, the study utilized primaryrndata that were collected through direct collection of milk from 24 Jennies from three selectedrnkebeles of Ada’a woreda; and secondary data were obtained from published literature forrncomparative purposes. Attempts were primarily made to analyze the physicochemicalrncomposition such as fat, total protein, lactose, minerals, vitamins C, acidity or pH, density, totalrnsolid and freezing points; and microbiological characteristics. Results indicated that thernAbyssinian donkey milk have close similarity with other donkeys milk previously investigatedrnelsewhere for most of the components. More importantly, the present findings confirmedrnAbyssinian donkey milk to have a very close similarity with human breast milk for protein,rnlactose, vitamin C, density, and acidity contents. However, it was found to have a significantlyrnhigher (p