Food safety problems, globally, resulted in illness of 600 million people death of 420,000 every years.rnSafe drinking water and basic sanitation are crucial to the preservation of human health, especiallyrnof children. This study assessed Knowledge, Attitude and Practice (KAP) of mothers of under-fivernchildren in Urban Injibara and Rural Kessa on Food Safety and Water, Sanitation and Hygienern(WASH) and evaluated household food access. A cross sectional study was performed during Marchrnto April 2020 across an urban Injibara and rural Kessa. A total of 235 mothers of under-five childrenrnwere considered in this study. A modified version of previously validated questionnaire was used torngather information on socio-demographics, food handling, water sanitation, personal hygiene,rnexisting water and sanitation facilities, water storage and treatment practices, waste disposal andrnhand washing practices. Household food access was also additionally evaluated using (HFIAS). Datarnwas analyzed by using SPSS version 22 through descriptive and inferential statistics. Among thernmothers, those aged 20 -40 years made up around 70% in rural and urban kebeles. There was highrnproportion of food secure (36% and 11%), mildly food insecure (38% and 46%) in urban than ruralrnkebeles. Regarding HFIAS difference between urban and rural households was not significantrn(p>0.1). Average knowledge regarding food handling was low both in urban (30%) and rural (22%)rnsettings. Knowledge of personal hygiene was also very low for urban (25%) and rural householdsrn(21%). Average water sanitation knowledge was also low (15% and 23% for urban and ruralrnhouseholds, respectively. Average positive attitude was also low both in the urban (36%) and ruralrn(32%) settings. Similarly attitude towards personal hygiene was also low (44% and 39% of urbanrnand rural respondents, respectively). Rural households (81%) practiced mainly open defecation.rnGenerally present study showed poor knowledge negative attitude and poor practice on foodrnhandling, personal hygiene water sanitation and WASH both in urban and rural settings. Regardingrnhousehold food access difference between urban and rural households was not significant. Safernpractice of water, sanitation and hygiene among mothers were affected by their education level andrnincome.