Study On Aspergillus Species And Aflatoxin Levels In Sorghum (sorghum Bicolor L.) Stored At Different Period And Storage System In Kewet Districts Northern Shewa Ethiopia.

Sorghum serves as staple food for over 100 million people in Sub-Saharan African countries. Itrnis the most important nutritional security crop and ranks third among major cereal crops inrnterms of area and production next to teff and maize in Ethiopia. However, sorghum is susceptiblernto contamination by fungal species mainly Aspergillui flavus and Aspergillui parasiticus speciesrnproducing mycotoxins, aflatoxins that have hepatotoxic and carcinogenic effects on humans andrnanimal. Study was conducted to assess Aspergillus species and aflatoxin levels in sorghumrn(sorghum bicolor L.) stored at different period and storage system. Thirty Samples werernanalyzed for aflatoxins contamination using HPLC with fluorescent detection and Aspergillusrnspecies were isolated and identified using culture media. About 56.7%, 16.7%, and 23.3% of thernsorghum samples were found to be contaminated with Aspergillus flavus, Aspergillus niger andrnAspergillus parasiticus, respectively. Total aflatoxin and AFB1 contamination occurred inrnsorghum with a maximum concentration range of 11.44 to 344.26μg/kg and 3.95 to 153.72μg/kg,rnrespectively. The levels of Aflatoxins detected in sorghum were within the range of 1.17 torn91.82μg/kg for AFB2, 3.22 to 52.02μg/kg for AFG2 and 9.87 to 139.64μg/kg for AFG1.rnSorghum stored for ≥ 2 years had high level of the aflatoxin B1 (52.19 μg/kg) followed byrnsorghum stored for < 12 months (38.24 μg/kg). Although storage period had resulted in nornsignificant difference in AFB2, AFG1, and AFG2 concentrations, AFB1 varied significantlyrn(P

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