Evaluation Of Isolates Of Pseudomonas Fluorescens As Biocontrol Agent Against Potato Bacterial Wilt Caused By Ralstonia (pseudomonas) Solanacearum

A total of 50 bacterial isolates of fluorescent pseudomonas were isolated fromrnpotato rhizosphere soil collected from different potato growing areas of therncountry. The isolates were screened In Vitro for their antagonistic activity againstrnthe pathogen, Ralstonia solanacearum on KB agar medium. Out of the 50rnisolates, only three (Pfs2, Pfw1, Pfwt3) showed a certain degree of inhibition tornthe growth of the pathogen. To test their antagonistic effect in a greenhouse, arnpot experiment was conducted using sterilized soil and potato tuber (CIPrn383032.15) that was susceptible to the pathogen. In the experiment,rnbacterization of potato tubers with the selected isolates i.e., Pfs 2, Pfw 1, Pfwt 3rnand Pfri (reference strain) significantly reduced the incidence of bacterial wilt byrn59.83%, and increased plant growth: plant height and dry weight by 76.89% andrn28.44%, respectively suggesting the importance of the isolates as plant growthrnpromoting rhizobacteria.rnKey words/Phrases: Potato, P. fluorescens, R. solanacearum, AntagonsticrnInteraction

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