Detection Of Plasnodiun Falciparun Sporozoites In Naturally Infected Anopheline Species Using Fluorescein Labelled Dna Probe And Pcr Procedures.

A dot blot assay to detect P. faicipaTum sporozoi tesrnfrom naturally infected An. gambiae s.l was developed. Indoorrnresting blood fed female An.gambiae s.l were collected fromrnMetehara, Zeway and Arbaminch. These mosquito specimens werernassayed for P.faicipaTum sporozoites using the hybridization.rnmethod with a fluorescein labelled PFRI oligomer and thernPolymerase Chain Reaction (PCR). A total of 198 fieldrncollected samples were assayed. P. faicipaTum sporozoi tes wererndetected from 4 samples; 2 from Zeway and the remaining 2,rneach from Metehara and Arbaminch by both hybridization andrn, ....rnpeR methods. Background signal in the hybridization ass~y,rngenerated from blood fed mosquitoes was cleared by keepingrnmosquito specimens in cages for 48 hrs after collection, sornthat the majority, if not all of the blood meal was digestedrnbefore killing and preservation. Results of this studyrnindicate that this method could be used for large scalernepidemiological studies.

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