Prevalence Of Bacterial Vaginosis Characterization Of Lactic Acid Bacteria Isolates And Patterns Of Antimicrobial Test From Women Vaginal Fluid In Addis Ababa
The human vagina is a complex integrated environment containing an abundancernof microorganism. Bacterial vaginosis (BV) is a disorder characterized by a reduction inrnor absence of Lactobacillus colonization, and overgrowth of several facultative andrnobligate anaerobic bacteria. BV has been reported as one of the most common vaginalrninfections in women at reproductive age from different part of the world. This study wasrnconducted to evaluate the prevalence of BV and some associated risk factors in AddisrnAbaba. Moreover the study examined the composition of lactic acid bacteria (LAB)rngenera isolated form vaginal specimen culture and the antimicrobial sensitivity pattern ofrnsome of the isolated LAB. Among 100 women randomly enrolled in this study a BVrnprevalence of 32% was found based on Gram-stain scoring of vaginal flora according tornNugent criteria. The prevalence of BV was higher among women at age group 31-40rn(44%), Christian women (34%), who are married (33%), non-pregnant (34%) and womenrnwho had a previous history of abortion (37%). Lactic acid bacteria (LAB) were isolatedrnfrom 70% of women. Out of 350 colonies which were isolated and characterized, 40%rnwere lactococci, 29% were lactobacilli, 23% were pediococci and 8% were leucnostoc.rnFrom a total of 60 lactic acid bacteria isolates that were subjected to antimicrobialrnactivity test, both the most frequent resistance and the least sensitivity result was notedrnfor Clindamycin (42% and 50% respectively), where as the most frequent sensitivity andrnthe least resistance was recorded for Vancomicine (80% and 20% respectively). Thisrnstudy has provided evidence for a considerable prevalence of BV in women. Therefore,rnthe health sector and other responsible bodies should give the concern to screening andrntreating women for bacterial vaginosis. On top of that creating the awareness among thernsociety will add its value on the public health profile of women and probably wouldrncontribute to reduce the risk of acquisition of other more serious STDs. Moreover, thernknowledge of lactic acid bacteria composition and pattern of antimicrobial activities ofrnhuman vaginal fluid would help to design better therapies and show future directions tornmaintain the healthy nature of human vaginal ecosystem.