Virological And Immunological Features Of Human Immunodeficiency Virus Type-l (hiv-l) Pre And Post Treatment Of Tuberculosis In Coinfected Patients

The association between HIVand Tuberculosis (I'B) is complex and bi- directional. Recentrnstudies demonstrated that TB accelerates the course of AIDS. In the present study lI'ernsought for virological and immunological features of HIV in TB co-ilifected individuals.rnThe study subjects are selected pom Ethio-Netherlands AIDS research project (ENARP)rncohort. 8 HIVITB co-ilifected individuals prior to diagnosis of TB, during and afterrncompletion of succes~rul TB chemotherapy and 7 CD4 matched HIV-1 ilifected patientsrnwithout the diagnosis of TB and with one year follow-up were involved As routinernlaboratory analysis viral load and CD4+cell countll'ere done at the time of blood samplerncollection. For this study, RNA was isolated pom plasma and the C2V3 region of HIV-frnenvelope gene was amplified by neste(l ,pO{YIII~I:a.ye, r:hain re,action, (PCR).l)ire.ctrn{ [ • , { • " < , " ( { { , { C " , ( [ , , ' sequencing was pelformed for all the PCR~ !Nsitn'e: fJl:O(/ucts. The, :HlVITB, :c(',itJjecledrn,~~~"':,,';,,,' ; ~;,,': ;;,,';;,rnindividuals showed a significantly elevate-p plf,lsl!lC[ .!fIV-} .. vi!,G:!: iori4' '(P'f-O; 05) .'durinffrntreatment of TB with steeper decline in CD4';: 'ck, l? ~'b[l ~, n,t ~'," : (: ;'~d". (5,): .,': li,o ;:l"'e ~er' ,, , : The ds:rn(synonymous nucleotide substitution) and dns (n~lJ. s:~llqrYilio~,J 'hIJie~tidi'; ,sl~l;'1itution)'rncomparison between the study groups and the' c'ol1ti'ols' elt inra'faJ (befor'e 'st'm't 'oj O'eat/llelit)'rnand ""f2 months later (qfter completion of TB treatment) didn't sholl' a significantrndiffirence between the two groups. The ratio of ds and dns between the two time points forrnboth groups was approximated to 1, which shows a positive but weak selection of evolutionrnof the virus. We conclude therefore that plasma H1V-f viral load is elevated during andrnafter treatment of active tuberculosis, but this may not be due to the presence of highlyrnreplicative HIV viruses. We recommend jilfure research should focus on isolating therndifferent HIV quasispecies so that one can see if there is a difference in HIV geneticrndiversity, diversification and diversification rate between the HIVITB and HIV only studyrnpopulation.

Get Full Work

Report copyright infringement or plagiarism