Sorghum (Sorghum bicolor (L) Monech) is among the leading cereal grains produced inrnAfrica and it is a staple food for millions of people in the continent. In Ethiopia, sorghumrnstands third in terms of area coverage after teff and maize, and second in terms of yield perrnhectare after maize. The spotted stem borer, Chilo partellus (Swinhoe) (Lepidoptera:rnCrambidae) is a major constraint limiting production of sorghum in Ethiopia. The presentrnstudy was conducted at Melkassa Agricultural Research Center experimental field with thernmain objective of screening sorghum genotypes which are resistant against Chilo partellus.rnTwo hundred one sorghum genotypes were planted at Melkassa Agricultural ResearchrnCenter in two replications in Randomized Complete Block Design. Twelve of theserngenotypes were selected for artificial infestation study in screen house pot experimentrndepending on stem borer percent infestation. The twelve genotypes were four fromrnresistance, four from intermediate and four from susceptible groups and they were planted inrnpot in screen house in completely randomized design in three replications. Forty mated C.rnPartellus adults were released in to the set up for artificial infestation of the genotypes.rnPopulation growth of C. partellus was also studied on the most resistant and the mostrnsusceptible genotypes. The results obtained indicate that there were high level of variabilityrnamong the genotypes tested both under natural infestation of field condition and artificialrninfestation of pot-screen house experiment. Significantly (P < 0.05) lower number of eggrnmasses, lower percent hatchability, lower percent pupation and lower percent survival of C.rnpartellus were recorded on A3 X 76 T1 # 23 suggesting that there is a possibility ofrndeveloping C. partellus resistant varieties. However, further evaluation of the sorghumrngenotypes, which are found in different adaptation areas are important and there is also arngreat need to develop varieties by breeders that can resist stem borer attack.rnKey words: Sorghum bicolor, spotted stem borer, genotypes, artificial infestation, screen