Seedling Biomass Seed Germination Responses And Cytology Of Hagenia Abyssinica (bruce) J.f. Gmel.

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Seeds (botanical fruit) of Hagenia abyssinicn (BnTc~)):I:.Qip.~l.,COllect~d fr6~i s~~eral site;rn, , , ,rnwere used to study germination response of the same species to different concentrations ofrngibberellic acid, kinetin, glucose, and vitamin B6 in the ranges of 10'3 M to 10" M. Therncontrols were treated with distilled water. Most treatments were found to increaserngermination percentages of the species, in the presence of great variation among therndifferent treatments. Of the chemicals used kinetin applied at 104 M were found to increasernthe germination percentages upto 80 %. Vitamin B6 applied at 10" M and gibberellic acidrnapplied at 104 M also increased seed germination next to kinetin applied at 104 M. Thernresults on germination percentages are discussed. In addition seedling biomass of H.rnabyssinica in time intervals (2, 4, and 6 months) were determined, and the results show thatrnthe shoots of the species grow at a faster rate but the root does not.rnSomatic chromosomes from the radicles were studied using aceto-orcein, toluidine blue, andrnfeulgen staining techniques. Pollen fertility was examined using the cotton blue lactophenolrnmethod. Although the chromosomes were rather small and difficult to study theirrnmorphology, it was possible to count them. The chromosome number for the species isrn2n =40. The number of nucleoli was determined using silver nitrate as a stain. Thernmaximum number of nucleoli observed was six, but the majority of the cells had only arnsingle nucleolus. Meiosis in pollen mother cells showed 20 bivalence, and meiosis wasrnfound to be highly regular. Examination on a total of 4,900 pollen grains revealed that 95-rn97 percent of the pollen were fertile.

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Seedling Biomass Seed Germination Responses And Cytology Of Hagenia Abyssinica (bruce) J.f. Gmel.

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