Gullele Botanic Garden contains diverse types of insects. The diversity,rndistribution, frequency, density, abundance and habitat association of these insects wererninvestigated using 60 quadrants which is located at different habitat types and transectrnwalk methods during the wet, intermediate and dry seasons. The mean comparisons ofrnGullele Botanic Garden showed that the presence of insects in large quantity both in wetrnseasons and natural habitats. From the total of 7593 insects collected, 64 families ofrninsects in Gullele Botanic Garden were recorded. In all seasons more evenness andrndiversity of insects were observed in the natural forest habitat. On the other hand, thernleast evenness and diversity of insects were observed in artificial forest habitats in allrnseasons. In the dry season, the minimum insect diversity and evenness was recorded in allrnforest habitats of Gullele Botanic Garden. Based on jaccard's similarity index, the highestrnsimilarity was observed between grassland forest habitat and mixed forest habitats inrnGullele Botanic Garden. On the other hand, the lowest similarity was observed betweenrnmixed forest habitat and artificial forest habitat. Natural forest habitat in Gullele BotanicrnGarden showed the highest Margalef’s Richness Index value. The result of ch-squarernanalysis showed that the highest habitat association was recorded in grassland habitat inrnwet and dry seasons, and mixed forest habitat in intermediate season. T test and analysisrnof variance (Anova) results for insect diversity revealed the presence of significancerndifference (variation) between seasons in Gullele Botanic Garden. Abundance of food,rnspecies of vegetation, and stability of the habitats, animal grazing and human activitiesrndetermined insect diversity at the study areas. Investment activities and human settlementrninside had the most detrimental effect to the insect diversity in the garden. During datarncollection in Gullele Botanic Garden, carefully observation showed that insects had bothrnpositive and negative effects for humans, plants, animals and insects.