Background: Hepatitis B infection is usually higher among health workers than the generalrnpopulation, particularly in the sub-Saharan region. WHO recommends vaccination for allrnhealth care workers (HCWs) against the highly infectious, blood borne virus: HBV.rnHowever, previous studies reported that knowledge of HCWs toward the hepatitis Brninfection, Hepatitis B vaccine and their vaccination coverage was low and vary among HCWsrnin different settings. rnObjective: This study was aimed to assess knowledge about HBV infection, HBV vaccinernand status of HBV vaccination among healthcare workers in TASH, Addis Ababa, Ethiopiarnfrom March 10 to September 10, 2020. rnMethods and materials: Hospital based cross-sectional study was conducted on 358 HCWsrnwho were selected randomly. Stratified random sampling method was used to collect the datarnusing self-administered questionnaire. Then, the data was entered into Epi-data 4.2 versionrnand was exported to Statistical Package for Social Science (SPSS) version 26 for analysis. Allrncovariates that showed p value < 0.25 in binary logistic analysis were further analyzed byrnmultiple logistic regressions to detect true predictors. rnResult: Among the health care workers, who participated in this study, 180 (51.4%) of themrnhad adequate knowledge on the HBV infection. Medical doctors were the mostrnknowledgeable among the HCWs, with interns [AOR=11.3(95% CI=4.5-28.6)], consultantrnphysicians [AOR=7(95% CI=3.1-15.9)] & resident physicians [AOR=3.5(95% CI=1.9-6.9)]rnbeing significant predictors of good knowledge. Vaccination coverage was 60% which wasrnpredicted by a shorter work experience [AOR=4.7(95%=1.4-5.8)]. rnConclusion: This study reveals that general knowledge level of HCWs about HBV isrninadequate and vaccine coverage is suboptimal. Thus, concerned stakeholders should devoternmore efforts to improve the HCWs awareness about the virus and role of its vaccine and alsornmore work to avail vaccine.