In a country like Ethiopia, where agriculture productivity levels are in the early stage of development, transferring advanced agricultural knowledge that used to solve inadequate farming activities is essential. To this end, the knowledge intermediaries’ (KI) motivation is the basic concept which is undermined. The purpose of the study was to examine the extrinsic and intrinsic factors that influence motivation of KI in agriculture. This study was answered the following research questions (I) what practice exist to transfer agricultural knowledge? (II) What extrinsic and intrinsic factors affect knowledge intermediaries’ motivation to knowledge transfer? To select the area of the study both Minister of Agriculture and Oromia Agricultural offices selected purposively. Whereas Adda Berga woreda was selected using two stage random sampling techniques from 180 woredas existed in Oromia region. All knowledge intermediaries, who satisfy the inclusion criteria made for this research in each organizational level was selected because of the population was small in size. The study population contained a total of 170 KIs from the three organizations. The basic evidences used in this study were collected from 152 knowledge intermediaries through structured questionnaires and 6 interview informants. To analyze the data, descriptive statistics was employed. The key findings of the study was that the extrinsic factors include, reward, recognition, training, working environment, transfer mechanism, role and stakeholder relationship identified as having significant impact on knowledge intermediaries’ motivation. And Intrinsic factors, including appreciation, work meaningfulness, trust, responsibility, significance and fairness identified as having significant impact on knowledge intermediaries’ motivation.