Ethiopian Biodiversity Institute (EBI) has been serving as preservation and conservation ofrnbiodiversity in Ethiopia since its establishment ,therefore, the research mainly emphasis onrnassessing the role of public relations activity in line with promoting Biodiversity of Ethiopiarnnationally and globally. In other words, the general objective of this study is to discover howrnthe existing public relations activities are implemented awareness creation purpose in EBIrncurrently. In principal, the researcher has used qualitative research method approach andrndocument analysis method. Data needed for this study were primary source of data andrnsecondary source of data. The data collection tool was in-depth individual interview duringrnthe study. The number of respondents which were selected for in depth interview were 20 byrnusing purposive sampling technique, in this case, researcher has analyzed the interviewrnresponses gathered from interviewees qualitatively. Besides this, secondary source of datarngained from library of the Institute and public relations department were reviewedrnqualitatively as well. The finding of the study showed that public relations practitioner hasrnnot carried out the research with regard to identifying the appropriate public relationsrnactivities which can be compatible with the nature of the EBI, therefore, PR activities and itsrntools were an obstacle to draw a picture about biodiversity of Ethiopia in the mind of public.rnGenerally, the concerned people could not get the significance information aboutrnBiodiversity as it is a part of human being from Ethiopian Biodiversity Institute lately. As arnresult, researcher has recommended that knowledge, attitude and practice analysis shouldrnbe considered during designing the strategic communication activity because strategicrncommunication is the key activity for awareness creation, besides this, corporate socialrnresponsibility activity should be applicable in face to face communication activity with targetrnaudience.