This research was designed to assess the effect of cold chain management practices onrnavailability of vaccines: the case of health centers under Addis Ababa city administration healthrnbureau. The study used a quantitative methodology with explanatory study design to generaternanswers to the research questions. Primary data were collected through questionnaire from coldrnchain responsible personnel in selected health centers. To determine the sample size for selectingrnhealth centers the researcher employed a sample determination formula developed by Cochranrnin 1963. In order to select the respondents, the study used probability sampling methodrnspecifically simple random sampling. Accordingly, an aggregate of 71 respondents were selectedrnand participated in this study and out of these, data were obtained from 62 respondents. Datarncollected through questionnaire were analyzed using descriptive statistics such as frequency andrnmean. As an aside, correlation was used so as to assess the relationship between cold chainrnmanagement practices and availability of vaccines. Multiple regression equation was used to testrnthe hypothesis of research. The findings of the study revealed that all the components of coldrnchain management practices and also availability of vaccines were rated average exceptrninformation system. The regression analysis revealed that storage and distribution practicesrnhave significant positive effect on the availability of vaccines. On the other hand technicalrncapacity and information system were found to have no significant effect on the availability ofrnvaccines. In order to improve the availability of vaccines in the health centers the researcherrnrecommend to FMOH and other concerning bodies to focus on all distribution and storagernpractices. Among the recommendations, using electronic databases such as Health CommoditiesrnManagement Information System (HCMIS), accelerating the transition of vaccine supply chainrnresponsibility from the Federal Ministry of Health to the Pharmaceuticals Fund and SupplyrnAgency, purchasing more cold chain equipments are some to mention. On top of that, thernresearcher recommend others to conduct similar research by expanding their scope to includernprivate and other governmental health facilities and also to find out actual vaccine wastage