BackgroundrnBlood is one of the integral components of body constituents. Blood transfusion is important therapyrnduring maternal bleeding, surgery and also a lifesaving procedure for anemic patients. Transfusionrnreaction and transfusion transmitted infections (TTI) are major problems associated with bloodrntransfusion. TTI is ranging from 8% up to 43%. The burden of transfusion transmitted infections inrndeveloped countries decline from time to time but it is still a challenge of developing countries likernEthiopia due to lack of adequate volunteer donors, testing material, good infrastructure and well trendrnpersonnel because of resource constraints. In addition to that there are insufficient data on seropostivityrnof TTI among blood donors in our setting.rnObjectivernTo assess the prevalence of transfusion transmitted infections among blood donors at Yirgalemrnhospital blood bank and Hawassa blood bank center, Sidama zone, SNNPRS Ethiopia.rnMethodologyrnInstitutional based cross-sectional study was conducted, convenient sampling techniques used tornaddress 384 donors and blood collection was done from health volunteers and replacement donorsrnfrom April-May, 2015 G.C at Yirgalem Hospital and Hawassa regional Blood bank. Data was coded,rncleaned, entered and analyzed by using SPSS version 21.Proportion, odds ratio and p-value werernused for analyses. P-value