Background: Body fluids serve as a medium for carrying nutrients and waste products.rnThese fluids are usually sterile, even if a single colony of bacteria may be significant torninvade and infect to body fluids and leads to morbidity and mortality. Assessing to thernprevalence of bacteria and testing their antibiotic susceptibility helps to provide effectiverntherapies, develop rational prescription writing and make policy decisions.rnObjective: The aim of this study was determining bacterial profiles and their antimicrobialrnsusceptibility patterns from body fluids at Tikur Anbessa Specialized Hospital, laboratoryrnAddis Ababa, Ethiopia.rnMethod: A cross sectional study was conducted from july 2015 to September 2015 and 384rnstudy participants were required. CSF, acsites, pleural and synovial fluid were collected andrncultured on Blood agar, MacConkey agar and chocolate agar and incubated according tornstandard conditions. All specimens were subjected to Gram Stain, WBC count and AFBrnimmediately following culturing. All culture positives were identified by gram stain andrnbiochemical tests using the standard procedure and antimicrobial susceptibility test wasrnperformed using Kirby-Bauer method. Data was entered and analyzed using SPSS versionrn22.rnResult: In this study 54/384(14. 0%) of bacteria were isolated from different body fluidsrnsuch as; Cerebrospinal fluids 264(68.8%), Pleural fluid 76(19.8%), Peritoneal fluidrn34(8.9%), and Synovial fluid 10 (2.6%) and using gram stains 41/384 (10.7%) of bacteriarnwere seen from the total of body fluids. Among these majority of body fluids 173(44.1%)rnhad abnormal white blood cell count (WBC) above 05 cells per mm3,rnout of themrn91(21.1%) had polymorphic feature. K. pneumonia 16.7 %( 9/32) were the most commonrnisolated bacterial following by coagulase negative Staphylococcus 15.0 %( 8/32). Amongrnall body fluid in CSF (31(57.4%) were the highest bacterial isolated. Out of 54 isolates,rn41(75.9%) of bacteria were showing multi drug resistance.rnGentamycin (76%) andrnerythromycin (59%) were the highest drug resistanc for gram-negative and gram-positivernrespectively.rnConclusion: The prevalence of bacterial isolates in this study was 14.0%. Frequency of singlernas well as multiple drug resistance was high. Over all 41/54, (75.9%) isolates had shownrnMultiple-drug-resistance of (MDRâ‰¥2) drugs