Background: Urinary tract infection (UTI) in pregnancy is associated with significantrnmorbidity for both the mother and the baby. However, little is known about UTI inrnpregnancy in the study area. Antimicrobial resistance among the pathogens that causernUTI is also increasing and is a major health problem in the treatment. Hence, properrninvestigation and prompt treatment are needed to prevent serious life threateningrncondition and morbidity due to UTI that can occur in pregnant women.rnObjectives: To identify the prevalent bacterial isolates that cause UTI and assess theirrnantibiotic susceptibility pattern among symptomatic and asymptomatic pregnant womenrnattending antenatal care in Mekele Hospital, Tigray Region.rnMethods: A cross sectional study was carried out from January to August 2014 on a totalrnof 168 pregnant women with and without symptoms of UTI that attended antenatal carernat Mekelle Hospital. Mid-stream urine samples were collected and inoculated ontornCystine Lactose Electrolyte Deficient medium (CLED). Colony counts yielding bacterialrngrowth of â‰¥105cfu/ml is regarded as significant bacteriuria. Pure isolates of bacterialrnpathogen were characterized by colony morphology, gram-stain, and standardrnbiochemical procedures. A standard method of agar disc diffusion susceptibility testingrnmethod was used to determine susceptibility patterns of the isolates.rnResults: In this study, the overall prevalence of UTI was 11.9%. Of this bacteriologicalrnscreening of midstream urine specimens showed that 11.3% and 15.4%, had significantrnbacteriuria in asymptomatic and symptomatic group respectively. Prevalence of UTI wasrnsignificantly associated with previous history of catheterization and urinary tract infectionrn(p