Back ground: Bloodstream infections are major health problems that lead to morbidity andrnmortality of patients unless treated with appropriate antimicrobial treatment. Drug resistance ofrnbacterial pathogens is challenging to alleviate sepsis. Blood culture is a gold standard techniquernwhich provides essential information for the diagnosis and proper medication.rnObjective: The Aim of study was to assess bacterial profile and antimicrobial susceptibilityrnpattern of blood culture isolates at Tikur Anbessa Specialized Hospital, Addis Ababa, EthiopiarnMethod: A cross-sectional study was conducted from April 2016 to September 2016. Usingrnconvenient sampling technique a total of 422 blood sample were collected from study participantrnwho were suspected for blood stream infection. Bacterial isolates were identified by colonyrnmorphology, gram staining reaction, biochemical tests such as catalase, coagulase, optochin,rnPYR, triple sugar iron agar (TSI), citrate utilization, urease, motility, Indole were used forrnbacterial identification and susceptibility to antibiotics was done using Kirby Bauer discrndiffusion technique method according to CLSI guidelines in Microbiology Laboratory of TikurrnAnbessa Specialized Hospital. The data were analyzed by using SPSS version 20.rnResult: About 66(15.6%) microbial were isolated. Out of this 64 (96.9%) were bacterial andrn2(3.03%) were fungal (Candida albicans) isolates. The predominant bacterial isolates werernS.aureus (22.72%) and K.pneumoniae (16.66%). Gram positive cocci were highly resistance tornPenicillin (86.7%), followed by Ceftriaxone (46.7%), and 40% were MRSA.While clindamycinrn(80 %), erythromycin (73.3%), and Trimethoprim-Sulfamethoxazole (66.7%) were effectivernantibiotics. Most of gram negative rods were highly resistance to ampicillin (85.7%), amoxicillinrnclavulanic acid (77.14%), Ceftriaxone (75%), and Ceftazidimen (62.8%). Nevertheless amikacinrn(97.1%), meropenem (91.5%), and ciprofloxacin (77.1%) were sensitive antibiotic. Most ofrnbacterial isolates (73.4%) were multidrug resistance to most frequently used antibiotics.rnConclusion: Overall, there was a high prevalence of blood stream infection with high resistancernrate to the most commonly used antibiotics. Hence, timely investigation of blood streamrninfection and regular surveillance of antimicrobial resistance pattern is important to reducernmorbidity and mortality due to blood stream infection.rnKeywords: Blood stream infection, Bacterial profile, Antimicrobial sensitivity pattern