Background: Urinary tract infection (UTI) is one of the most common infections encounteredrnand treated worldwide. UTI has been reported to be around four times higher in diabetics compared tornnon-diabetic patients. Since diabetic patients are at a high risk of development of UTIs, it isrnrecommended that special attention is paid to them, especially for the management of bacterialrnUTIs.rnObjective: The aim of this study was to determine prevalence of significant bacteriuria andrnassess drug susceptibility pattern of the bacterial isolates in diabetic patients.rnMaterials and Methods: A cross sectional study was conducted in Yekatit 12 Hospital MedicalrnCollege from April to July 2015. . In this study, 246 patients diagnosed with diabetes mellitusrnwere recruited consecutively. A standard questionnaire was used to collect information regardingrnthe socio-demographic status and clinical history of the study participants. Mid-stream urine wasrnused for chemical analysis, microscopy and microbiological isolation and identification ofrnbacteria. Standardized disc diffusion method was used for the determination of antibioticrnsensitivity testing. Data were entered into and analyzed by SPSS version 20.rnResults: Among the 246 study participants, 150 (60%) of them were females and 96 (40%) werernmales. The mean age of the study population was 51.5 years. Pyuria was detected in 27 (11%) ofrnthe urine specimens examined microscopically. Of the 246 urine specimens cultured on CLEDrnand blood agar plate, 17(6.9%) of them showed significant bacteriuria. Among the 17 bacterialrnisolates, E. coli was the most frequently isolated one, accounting 10 (53%), followed byrnKlebsiella species 4 (21%). All the isolates showed 100% sensitivity to nitrofurantoin, exceptrnProteus species, which exhibited resistance to the antibiotic. The multidrug resistant rate of thernisolates in our study was 31.6%rnConclusion and Recommendation: The overall prevalence of significant bacteriuria in ourrnstudy was 6.9%. Even though Proteus species showed resistance to nitrofurantoin, all thernremaining isolates were 100% sensitive to the antimicrobial. Use of antibiotics for treating UTIrnin DM patients should be supported with periodic culture and drug susceptibility test studies in arngiven geographical location.