Effect Of Specimen Handling On Quantitative Hiv-1 Viral Load Measurement At Saint Paul Hospital Millennium Medical College Addis Ababa

Background: Proper specimen handling prior to quantification of plasma HIV-1 RNArnviral load is important, since some reports suggest that variations in specimen handlingrnmay affect detection and quantification of plasma RNA. However, there is limitedrnevidence on the effect of time of plasma separation, storage, freeze-thawing and dilutionrnon the HIV-1 RNA viral load.rnObjective: To determine the effect of sample management on HIV-1 RNA viral loadrnmeasurement in St. Paul Hospital Millennium Medical College from April to July 2019.rnMethods: Experimental study design was conducted in St. Paul Hospital MillenniumrnMedical College from April to July 2019 GC in people living with HIV. Whole bloodrnsample was collected into two EDTA test tubes from 88 eligible participants. The viralrnload test was done by Abbott m2000sp/rt analyzer. Data was entered into Microsoft excelrnand analyzed by SPSS version 20. Repeated measure analysis of variance was used torncompare HIV RNA viral load mean difference between different time of plasmarnseparation, storage, freeze-thawing and dilution. Post-hock analysis was employed tornlocate the place of significant difference. Level of significance was set at 5%.rnResults: There was significant HIV-1 RNA viral load log mean difference between plasmarnseparation time at 6 hours (hrs) and 24hrs (p

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