Background: Otitis media is a major health problem and occurs with a high incidence andrnprevalence in both developed and developing countries. The burden and populationrndemographics of otitis media differ greatly between developed and developing regions. SubrnSaharan Africa account for most deaths from complications arising from otitis media.rnObjective: The objective of this study was to determine the bacterial isolates and their drugrnsusceptibility patterns from patients who had ear infection at Arsho Advanced MedicalrnLaboratory.rnMethods: Cross-sectional study was conducted at Arsho medical laboratory, Addis Ababa,rnEthiopia from January to April 2019. By using an aseptic technique ear discharge samples wererncollected by a sterile swab and inoculated on Blood Agar, Chocolate Agar and Mac Conkey'srnmedia. Isolated bacterial species were identified and drug susceptibility testing was performed byrnusing the Vitek 2 system (bio-MÃ©rieux). Data were entered and analyzed by using SPSS versionrn20 software and P-value of < 0.05 was considered statistically significant.rnResults: Out of 422 samples processed, bacterial pathogens were isolated fromrn19.5% (82) samples. From the total bacterial isolated 78% were gram negative bacteria and thernpredominant isolate was Pseudomonas species and Proteus spps 21.9 % each followed byrnKlebsiella spps 20.3%. Among gram negative bacteria isolates 46.9 % had multiple antibioticrnresistant patterns. Pseudomonas aeruginosa 92.4% and proteus vulgaris had 80 % werernresistant against the commonly used antibiotics Ampicillin & Amoxicillin.rnConclusion: Majority of the bacterial isolates were multidrug resistant, hence, efforts to isolaternmicroorganisms and determine the susceptibility pattern should be strengthened to improve therntreatment outcome of otitis media instead of the usual trend of empirical treatment.