Background: Pulmonary mycosis is a systemic fungal infection that occurs when fungi causeâ€™srndisease of the lungs. Fungal pulmonary infection has been emerging recently due to widely usedrnbroad-spectrum antibiotics and steroids, immune deficiency or suppression in tuberculosis,rnpatients are easily vulnerable to opportunistic fungal infections.rnObjective: To determine the distribution of pulmonary fungal isolates among pulmonaryrntuberculosis presumptive patients in the sputum sample by conventional culture.rnMethods: A cross sectional study was conducted at Saint Peters specialized hospital on 636rnsputum samples from presumptive tuberculosis patients. Early morning sputum samples wererncollected aseptically and Presence of pulmonary Tuberculosis was screened by Xpert MTBRif/rnassay. All sputum samples were cultured using Sabrouad dextrose agar and Brain heartrninfusion agar medium incubated at 250C and 370C for 4 weeks respectively for each sputumrnsamples. Identification was performed by noting; the growth form and rate of growth, surfacernand reversed coloration. Yeasts were identified by conventional biochemical tests andrnassimilation characteristics. Chromo agar medium used for Candida species and urease used forrnCryptococcus neoformance and the rest were non-yeast species. Data analysis was carried outrnusing SPSS version 23 software.rnResults: The distribution of pulmonary fungal isolates among pulmonary tuberculosisrnpresumptive patients was at 75.9% of the infected patients, male patients accounted for 51.4%)rn%, whereas female patients accounted for 48.6%.Yeast isolates (82%) and mold isolates werern(18%). Among 163 tuberculosis (TB) cases 127(77.9%) were co-infected with pulmonary fungalrnpathogen.rnConclusion: High distribution of fungal isolation (75.9%) was obtained in patients withrnrespiratory symptoms. We recommend for policy makers to conduct further studies and considerrnthe need of fungal screening in these patients.