Background: Several factors like altitude, age, sex, pregnancy, socioeconomic status, life style rnand race influence hematological reference interval (RIs), which are critical to support appropriaternclinical decisions and to interpret laboratory data in research. Currently there are no wellestablishedrnRIsrnforrncordrnbloodrnhematologicalrnparametersrnofrnnewbornsrnin Ethiopia.rnrnObjective:rnTornrngenerate reference interval for umbilical cord blood hematological parameters ofrnnewborns at St Peter Specialized Hospital Addis Ababa, Ethiopia.rnMethod: A cross-sectional study was conducted from January 1 to March 31, 2019 on healthy,rnterm newborns (37-42 weeks) with normal birth weight born to apparently healthy pregnantrnmothers who had met the eligibility criteria. From a total of 139 newborns, 2-3ml cord blood wasrnimmediately collected from the clumped cord using EDTA tube. The samples were analyzed usingrnSysmex KX 21 hematology analyzer. Data was entered and the 2.5rnthrn and 97.5rnthrn percentile (upperrnand lower reference limit) were determined using non parametric method by SPSS version 23. Thernnon-parametric independent Mann-Whitney U test (Wilcoxon rank-sum test) was used to comparernthe distribution of the parameters between genders, modes of deliveries and gestational age groups.rnResult: The combined reference interval for umbilical cord blood hematological parameters ofrnnewborns with the median and 95% reference values were as follows for WBC= 12.4 [6.55-19.35],rnRBC= 4.51 [3.55-5.52], HGB= 15.80 [12.41-19.65], HCT= 45.9[37.9-56.3], MCV=102.10[83.90111.55],rnrnMCH= 35.30 [29.35-39.10], MCHC= 34.3 [32.3-37.40], PLT= 236 [146-438], LYM=rn37.5% [16.6-63.0%], MXD= 7.9%[1.65-15.75%], NEU= 53.7%[30.3-78.3], RDW= 15.6[12.019.0],rnrnPDW=11.0[9.1-15.7] and MPV= 9.4[8.1-11.8]. The current study found no significance rndifference between genders, except RDW (P=0.01) and gestational age group, but there was rnsignificance difference for WBC (p=0.007), RBC (p=0.018) and Absolute NEU (p=0.001).rnConclusion: The values obtained from our study provide reference intervals for somernhematological parameters in healthy newborns of Addis Ababa and its surrounding specialrnweredas. However, the results need to be confirmed by larger samples from different centersrnthroughout the country.