Magnitude And Drug Resistance Profile Of Extended Spectrum -lactamase (esbl) Producing Gram Negative Bacteria From Nasal Swabs Taken From Health Care Workers And Inanimate Objects At Tikur Anbessa Specialized Hospital Addis

Medical Laboratory Sciences Project Topics

Get the Complete Project Materials Now! »

Backgroundrn:rnHospital environment, inanimate objects and healthcare workers are likely to getrncolonized with diverse group of microbial agents. Infections caused by gram negative bacteriarn(GNB) are causing morbidity and mortality worldwide, gram negative bacteria which produce β-rnlactamase can result in multidrug resistance. The production of extended-spectrum β-lactamasesrn(ESBLs) is an important mechanism which is responsible for the resistance to the thirdgenerationrncephalosporin.rnrnrnObjective: The objective of this study was to determine the magnitude and drug resistancernprofile of ESBL producing gram negative bacteria isolated from nasal swabs taken from healthrncare workers (HCWs) and inanimate objects at Tikur Anbessa Specialized Hospital.rnMethods: Both prospective and retrospective cross sectional study were conducted fromrnDecember 2018 to February 2019.Isolates from samples collected from an on-going PhD projectrnwere used for the purpose of the current study. The samples were taken from nasal swabs ofrnhealth care workers and inanimate objects. About 105and 216 isolates were randomly selectedrnfrom health care workers and inanimate objects respectively for further analysis. Biochemicalrntests for identification and antimicrobial susceptibility test was done by disc diffusion method.rnScreening of ESBLs was done using Cefotaxime (30ug, BD), Ceftazidime (30ug, NJ54, QB14),rnceftriaxone (30ug BD) discs. A combined disk diffusion test was used as confirmatory test. Therndata were analyzed using SPSS software (version 20) and descriptive statistical tests werernperformed rnResults: Out of 105 isolates, 5 (4.8%) isolates were identified as Extended Spectrumrnβlactamasesrnrn(ESBLs) producers, out of theses80% were Klebsiella spp (75% Klebsiellarnrhinoscleromatis, 25% Klebsiella pneumoniae) and 20% were Entrobacter cloacae. Three (60%)rnESBL producing bacterial isolates were identified from Laboratory personnel and 2 (40%) werernfrom Medical doctors. From environmental samples, 33/216 (15.3%) isolates were found to bernESBL producers based on the confirmatory test (combined disk method). Different ESBLrnproducing gram negative bacteria, were isolated from the various inanimate objects of TASHrnincluding, Klebsiella ozaenae, Klebsiella oxytoca, Klebsiella pneumoniae, Klebsiellarnrhinoscleromatis,Citrobacter_spp, Escherichia coli, Serriatia_spp and Acinetobacter_spp. ESBL rnproducing gram negative bacteria were found to be 100% resistant to ceftazidime andrnceftriaxone. rnConclusion: It is worrisome to detect ESBL producing gram negative bacteria from therninanimate objects of TASH and the nasal cavity of some HCWs, calling for systematic screeningrnof inanimate objects for ESBL and other multidrug resistant bacteria in the hospital.rnFurthermore, strengthening the infection prevention practice is vital to halt transmission of thesernnoxious bugs.

Get Full Work

Report copyright infringement or plagiarism

Be the First to Share On Social



1GB data
1GB data

RELATED TOPICS

1GB data
1GB data
Magnitude And Drug Resistance Profile Of Extended Spectrum -lactamase (esbl) Producing  Gram Negative Bacteria From Nasal Swabs Taken From Health Care Workers And Inanimate Objects At Tikur Anbessa Specialized Hospital Addis

243