Introduction: Multidrug resistant (MDR) bacteria constitute a global emergency. Of whichrnextended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-PE) havernbecome the most common type. ESBL-PE are important causes of serious infections in therncommunity as well as in hospital settings. rnObjective: To determine the magnitude and patterns of resistance of extended Spectrum Î²lactamasernrnproducing Enterobacteriaceae isolates from patients attending Tikur AnbessarnSpecialized Hospital, Addis Ababa, Ethiopia.rnMethods: A cross sectional study was carried out to identify and characterize antimicrobialrnsusceptibility pattern of Enterobacteriaceae isolates from TASH between July 2018 tornNovember 2019. A total of 287 Enterobacteriaceae isolates were isolated from different rnspecimens: blood, urine, wound site discharge, body fluids were analyzed using blood agar,rnMacConkey, Xylose lysine deoxycholate and salmonella shigella agar(SSA). Bacterialrnspecies identification was carried out using standard biochemical tests. Antimicrobialrnsusceptibility testing and detection of ESBL production was performed using standardrnantibiotics on Muller Hinton agar using disk diffusion method and a combination disc methodrnfollowing 2018 CLSI guideline.rnResults: The overall magnitude of ESBL production was 62.7% (n=180/287). Female hadrnhigher proportion of ESBL positivity (66.1%, n=84/127) than male patients (60%, n=96/160).rnAmong the Enterobacteriaceae isolates tested, 81 (72.3%) of the 112 K. pneumoniae, 21rn(65.5%) of 32 K. oxytoca and 61 (58.7%) of 104 E.coli were ESBL producers. ComparablernESBL production by 158 (64.5 %) of 245 isolates from in patients and 22 (52.4%) of 42rnisolates from outpatient was observed. There was no significant association betweenrnhospitalization and ESBL production (P=0.136, OR=0.606, 95 % CI). However, when werncompare ESBL production of isolates from different wards a high ESBL production wasrnnoted among isolates from pediatrics ward 57(31.7%) of 180 isolates. The ESBL producingrnisolates showed highest and lowest level of resistance to ampicillin and amikacin 96.9% andrn9.0 %, respectively. Two-hundred sixty four (91.9%) of the 287 isolates had MDR pattern. rnConclusion: Significantly all isolates of Enterobacteriaceae showed higher rate of resistancernto all tested antibiotics. But amikacin was found most effective for ESBL producers and nonproducers.rnrnHigh rate of ESBL and high numbers of MDR organisms were isolated amongrnEnterobacteriaceae. Therefore, strengthening of monitoring antibiotics resistance in additionrnto regulation of the spread of resistance genes and preparing strong policies for monitoringrnutilization of antibiotics is required.