Extended-spectrum -lactamaseproducing Gram Negative Bacteria From Gowns And Mobile Phones Of Tikur Anbesa Specialized Hospital Health Care Workers

Background: Extended spectrum β-lactamases are rapidly evolving group of enzymes produced byrncertain bacteria that are able to hydrolyze cephalosporin, penicillin and monobactam. Mobile phones andrngowns serve as reservoir of ESBL producing microorganisms that could be easily transmitted to thernhealth care workers hands from. Hospital-acquired infection is an increasing global concern for patientrnsafety and it affects more than 25% of the total health care admissions in developing countries. rnObjective: The aim of the study was to determine the burden of extended spectrum β- lactamasernproducing gram negative bacteria and assess their antimicrobial susceptibility patterns isolated fromrnmobile phones and gowns of health care workers at Tikur Anbesa specialized hospital. rnMethods: A laboratory based cross sectional study was done from January 2019 to April 2019. A total ofrn572 paired samples were collected from mobiles and gowns. Bacterial isolates were screened for ESBLrnproduction using CHROME agar ESBL and confirmed using cefotaxime, cefotaxime-clavulnic acid andrnceftazidime, ceftazidime –clavulnic acid method according to clinical laboratories standard institutes. rnResults: Overall, the magnitude of gram negative bacteria isolated from mobile phones and gowns wasrn454/572(79.4%) and 477(83.4%) respectively, and out of 454 gram negative bacteria isolated fromrnMobile phones, 48/572 (8.3%) were extended spectrum β-lactamases producers. About 54/572(9.4%)rnhealth care workers gowns were also contaminated with extended spectrum β-lactamases producingrnbacteria. K .pneumoniae, Acinetobacter, E.coli and Citrobacter were the most dominant isolates. ESBLrnproducers were highly resistant to ampicillin (81%-100%), cotrimoxazole (67%-100%) andrnchloramphenicol (67-100%) and highly sensitive to meropenem (96%-100%), imipenem996-100%) andrnpiperacillin-tazobactam(90%-100%). rnConclusion: Health care workers mobile phones and gowns were contaminated with pathogenic bacteria.rnSince gowns and mobile phones could serve as a vehicle and a reservoir for pathogenic microorganisms,rnhealth care workers should be aware on the public health risk of hospital-acquired infection andrnappropriate interventions mechanisms should be in place to reduce the burden and cross transmission.

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